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Composition of modified Basch’s medium Basch’s medium
| Basch’s medium (Basch, 1981) | Working concentration |
|---|---|
| Basal Medium Eagle (Invitrogen catalogue no. 21010) | to final volume |
| Lactalbumin hydrolysate | 1 g/L |
| Glucose | 1 g/L |
| Hypoxanthine | 0·5 μM |
| Serotonin | 1 μM |
| Insulin | 8 μg/ml |
| Hydrocortisone | 1 μM |
| Triiodothyronine | 0·2 μM |
| MEM vitamins (Invitrogen cat. 11120-052) | 0·5× |
| Schneider’s medium | 5% |
| Foetal bovine serum | 10% |
| Hepes | 10 mM |
| Antibiotic/antimycotic (Invitrogen cat. 15240-062) | 1× |
