Nectin 4 gene overexpression in ovarian cancer. A, Reverse transcriptase–polymerase chain reaction (RT-PCR) of nectin 4 in ovarian cancer (C1–C5) and normal ovarian tissue (N1–N7). MCF7 cell line, positive control. −RT, no reverse transcriptase; −RNA, no RNA. B, Quantitative RT-PCR analysis of nectin 4 expression in immortalized NOSE and ovarian cancer cell lines. Results expressed as fold-change compared with the lowest expressing cell line (1816-575). *OVCAR3 messenger RNA was increased 454-fold over the lowest expressing cell line.