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. Author manuscript; available in PMC: 2011 Feb 20.
Published in final edited form as: Am J Clin Pathol. 2010 Nov;134(5):835–845. doi: 10.1309/AJCPGXK0FR4MHIHB

Figure 2.

Figure 2

Nectin 4 protein expression in ovarian cancer. A, Western immunoblotting of ovarian cancer cell lines and immortalized normal ovarian surface epithelial (NOSE) cell lines. Nectin 4 appears as an approximately 66-kDa band. β-Actin was used as a loading control. B, Flow cytometric analysis of ovarian cancer and immortalized NOSE cell lines for nectin 4 (top row), nectin 1 (middle row), and ADAM17 (bottom row). Nectin 1 expression indicates that trans-heterodimers may form between nectin 4– and nectin 1–expressing cells. Expression of ADAM17 indicates that nectin 4 may be cleaved from the surface of the cells and may be detectable in patient serum samples. Dashed line, IgG control stain; solid line, nectin 4, nectin 1, or ADAM17.