Immunohistochemical staining for nectin 4 of ovarian cancer tissue microarrays. A, Staining and grading examples of tissue microarray (TMA) samples. IgG, control primary stain. +1, <10% of cancer cells staining; +2, 10%–50% of cancer cells staining; and +3, >50% of cancer cells staining. B, Percentage of TMA cases positive for nectin 4 by ovarian cancer subtype. C, Percentage of TMA cases positive for nectin 4 by Silverberg grade. D, Kaplan-Meier survival curve of TMA patients measured in days. Red, staining score 0; green, staining score +1; blue, staining score +2; orange, staining score +3.