Skip to main content
. 2011 Feb 15;25(4):373–384. doi: 10.1101/gad.2004811

Figure 4.

Figure 4.

Quantitative analysis of AS by RNA-seq reveals that knockdown of SmB/B′ leads to increased skipping of alternative exons. (A) Western blots indicate that SmB/B′ (top panels) or SRSF1 (bottom panels; also known as SF2, ASF, SFRS1) were efficiently depleted by siRNA transfections. (NT) NT siRNA. Serial dilutions of protein extract indicate that the blots are semiquantitative. (B) The percentage of alternative exons (left) or constitutive exons (right and inset) showing changes in inclusion levels upon knockdown of SmB/B′ or SRSF1 when compared with inclusion levels in cells transfected with a control NT siRNA are shown in a bar graph. (C) Distinct effects of SmB/B′ and SRSF1 knockdowns. The percent exon inclusion values in the control NT, SmB/B′, and SRSF1 knockdowns are shown for 268 alternative exons found to have a ≥30% inclusion change (increased skipping) in the SmB/B′ knockdown when compared with the control (NT). The AS events are ordered from top to bottom by the absolute difference in percent inclusion between the SRSF1 knockdown and the control (NT).