Figure 6.

Characteristics of alternative exons affected by knockdown of SmB/B′. (A,B) Cumulative distribution function (CDF) plots of 3′ss scores (A, top), 5′ss scores (A, middle), and exon lengths (B) for alternative and constitutive exons profiled by RNA-seq (Fig. 4). Alternative exons that show a pronounced increase in skipping (≥30%) upon knockdown of SmB/B′ are plotted separately from other profiled alternative exons, as shown in the bottom panel of A. (C) CDF of the fold change in overall mRNA transcript level (log₂ scale; SmB/B′ knockdown vs. control) of transcripts containing AS events that are more skipped upon knockdown of SmB/B′ compared with the control knockdown. Transcripts containing AS events that introduce a PTC upon exon inclusion or skipping or that do not introduce a PTC (No PTC) are plotted separately, as shown in the legend below the plot. (alt) Alternative; (const) constitutive.