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Antagonism of prostaglandin E2 (PGE2)-induced Ca2+ flux by L-826266 (10 µM) in human recombinant (A) EP1/EP3 and (B) EP2/EP4 receptor systems. Each receptor was co-transfected with chimeric G-protein into HEK-293 EBNA cells; Ca2+ flux was measured by Fluo-4 fluorescence (fluorimetric imaging plate reader assay). Responses were normalized to the peak signal for 1 µM PGE2. Vertical bars show SEM (n= 3).
