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Estimation of pA2 values for prostanoid antagonists on guinea-pig aorta using a Schild protocol (protocol B, Figure 2B). (A) and (B) EP3 assay: log concentration–response curves for 17-phenyl PGE2 in the presence of L-798106 and L-826266 (180 min treatment); PE, phenylephrine priming. BMS-180291 (300 nM) was present for all tests. (C) Schild plots: TP agonist was U-46619; EP3 agonist was 17-phenyl PGE2. Vertical bars show SEM (all n= 4).
