Figure 2.

ATP acts itself and A1 adenosine receptors are not involved in the effects of ATP. In addition to DNQX, AP5 and bicuculline, cadmium was added to the superfusion ACSF to block voltage-gated calcium channels. ATP was pressure ejected from a pipette four times. Amplitudes of ATP-evoked currents were expressed as percentages of the initial reference value PRE. Shown are means ± standard error of the mean. (A) After the second ATP application, solvent (SOL; n= 3) or the ecto-nucleotidase inhibitor ARL67156 (n= 8) was superfused. The original tracings were recorded in a slice with ARL67156 superfusion at time points 1 and 2. (B) After the second ATP application, solvent (SOL; n= 3) or the A1 receptor antagonist DPCPX (n= 4) was superfused. The original tracings were recorded in a slice with DPCPX superfusion at time points 1 and 2.