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. 2011 Feb;162(4):974–988. doi: 10.1111/j.1476-5381.2010.01106.x

Figure 3.

Figure 3

The stable ATP analogue GTPγS does not affect the pattern of ATP-evoked currents. In addition to DNQX, AP5 and bicuculline, cadmium was added to the superfusion ACSF to block voltage-gated calcium channels. The patch clamp pipette contained GTP or GTPγS. (A) The mGluR agonist DHPG was pressure ejected from a pipette three times. In the presence of GTP in the patch-clamp pipette (n= 7), the DHPG-evoked charge transfer remained constant (charge transfer was calculated for the 50-s period following DHPG application). GTPγS in the patch-clamp pipette (n= 7) prolonged the effect of the first DHPG application (thereby increasing charge transfer), and diminished the effects of the further DHPG applications. * Indicates significant difference from GTP (P < 0.05). (A2-A3) Original tracings of DHPG-evoked currents in the presence of GTP or GTPγS. (B) ATP was pressure ejected from a pipette three times. In the presence of GTP in the patch-clamp pipette (n= 5), the ATP-evoked charge transfer remained constant (charge transfer was calculated for the 50-s period following ATP application). The ATP effect was similar in experiments with GTPγS in the patch-clamp pipette (n= 5). (B2-B3) Original tracings of purinergic currents in the presence of GTP or GTPγS.