Figure 9.
ATP does not suppress sIPSCs recorded in the presence of cadmium and thapsigargin and calcium-free ACSF. In addition to DNQX and AP5, cadmium (10−4 M) and thapsigargin (10−5 M) were present in the calcium-free superfusion ACSF. Figure 8 shows the first part of the experiment, during which ACSF with normal calcium concentration was superfused. (A–C) The frequency, amplitude and cumulative amplitude of cadmium sIPSCs were evaluated in 10-s periods and expressed as percentages of values during the initial reference period PRE. Means ± standard error of the mean of 12 (SOL) and 10 (RIM) experiments. (D) An original tracing recorded in the presence of solvent (the period between the dashed lines was not evaluated in A–C).