Table 3.

Samples Used to Obtain Provirus Sequences and Their Copy Numbers

Dog-integrant	Nearest genea	Cell source	Total no. of readsa	No. of junction readsb	% CD18+ cells	LM-PCR copy no.c	qPCR copy no.	qPCR/LM-PCRd
FD1-1	ABI2	Leukocytes	151	4	5.8	1.54 × 10−3	1.20 × 10−3	0.78
FD1-2	MECOM	Leukocytes	151	2	5.8	7.68 × 10−4	6.80 × 10−4	0.89
FD1-3	NBN	Leukocytes	151	1	5.8	3.84 × 10−4	1.60 × 10−3	4.2
FD1-3	NBN	Lymphocytes	84	2	27	6.43 × 10−3	1.10 × 10−3	0.17
FD1-4	SYNJ2BP	Leukocytes	151	7	5.8	2.69 × 10−3	2.20 × 10−2	8.2
FD1-4	SYNJ2BP	Lymphocytes	84	9	27	2.89 × 10−2	1.80 × 10−1	6.2
FD1-4	SYNJ2BP	Neutrophils	114	2	3.7	6.49 × 10−4	1.60 × 10−2	25
FD2-1	FUT8	Neutrophils	61	1	6.7	1.10 × 10−3	1.10 × 10−3	1.0
FD2-2	PAX5	Neutrophils	61	1	6.7	1.10 × 10−3	1.20 × 10−5	0.011
FD3-1	MTCP1	Leukocytes	181	2	5.1	5.64 × 10−4	1.10 × 10−4	0.20
FD3-1	MTCP1	Lymphocytes	81	1	18	2.22 × 10−3	2.50 × 10−4	0.11
FD3-2	GLYATL2	Leukocytes	181	1	5.1	2.82 × 10−4	3.00 × 10−4	1.1
FD3-2	GLYATL2	Neutrophils	60	3	4.7	2.35 × 10−3	1.70 × 10−5	0.0072
FD3-3	MECOM	Neutrophils	60	1	4.7	7.83 × 10−4	6.00 × 10−4	0.77
FD4-1	ALOX5AP	Lymphocytes	79	1	25	3.16 × 10−3	3.40 × 10−4	0.11
FD4-1	ALOX5AP	Neutrophils	95	1	2.8	2.95 × 10−4	3.50 × 10−5	0.12
FD4-2	RNF38	Neutrophils	95	1	2.8	2.95 × 10−4	2.80 × 10−4	0.95

^aNumber of sequence reads obtained from that cell source in that animal.

^bNumber of sequence reads obtained for that specific provirus junction.

^cEquals [(no. of specific junction reads)/(total no. of reads with junction sequence)] × [(% CD18+ cells)/100].

^dqPCR copy no./LM-PCR copy no.