Table I.

Primers and synthetic peptides used in this study

Primers
Purpose	Name	5′ to 3′ sequence	Size (bp)
Full-length tprK amplification and sequencing	tprK-S1	ACCGGGCATGAATTTTCTTT	1593
	tprK-As1	CCATACATCCCTACCAAATCA
	tprK-int-As2	CCTACCCGCTGATACACCAC
	M13rev2	CAGGAAACAGCTATGAC
	M13for2	GTAAAACGACGGCCAG
tprK fluorescent fragment length analysis3	FAM-V1-S	GTGGGCTCAGGTTTCGTTC	187
	V1-As	CGCATAGACATTCCCCTCAC
	FAM-V2-S	GGGGCTCACGTTTGATATTG	183
	V2-As	CCGGTGAGCTCCACTTTAAT
	FAM-V3-S	GAGCGTACGCGTGAAGATG	166
	V3-As	TAGCAGCCAGAGCACACAGA
	FAM-V4-S	CTTTGGGGTCTGTGTGCTCT	112
	V4-As	AACGATACCCCAACGTCAAC
	FAM-V5-S	TTGGGGTATCGTTGGTTCTC	173
	V5-As	CCCAAATCAAGACCCTCAAG
	FAM-V6-S	AAACCAAGGGGTCTGATCCT	188
	V6-As	TAGACGATACGAACCCCAGA
	FAM-V7-S	TGGGTGAGTATGGTTGGGTTA	159
	V7-As	GCCGAATCTCCACCTTCTCT
Real-time qPCR	TP0574-S	CGTGTGGTATCAACTATGG	313
	TP0574-As	TCAACCGTGTACTCAGTGC
	RT-tprK-S	AGTTTGCGTCTAACACCGACTG	410
	RT-tprK-S	TCGCATGGCCATGTTGAGAAAT
	rHPRT-S	TGATAGATCCATTCCTATGACTGTAGA	265
	rHPRT-As	GGGTCCTTTTCACCAGCAG
	rIFN-γ-S	TTCTTCAGCCTCACTCTCTCC	224
	rIFN-γ-As	TGTTGTCACTCTCCTCTTTCC
Synthetic peptides
Purpose	Name	NH2- to COOH- terminus sequence	Size (aa)
ELISA	ChicC1-V6	VHYKVLKARAQAPAAVPAAADDIYF	25
Immunization	ChicC2-V5	ASQASNVFQGVFLTTPMQKDDC4	22
	ChicC2-V6	MPVHYKVLKARARAGAAVPAAADDIYFPV	29

¹tprK-S/As primers were used for both full-length ORF amplification and sequencing after cloning.

²Sequencing primer. M13for and M13rev primers are PCR-II TOPO vector primers flanking the cloning site.

³All sense primers are 5′-fluoresceine (6-FAM) labeled.

⁴The final C residue was added during synthesis to allow conjugation with the KLH carrier.