Figure 2.

Effect of deletion of the β2 subunit on subsets of high-affinity [¹²⁵I]epibatidine binding sites and [¹²⁵I]-α-conotoxin MII binding. [¹²⁵I] Epibatidine (0.2 nM) was incubated with sections derived from β2^+/+, β2^+/−, and β2^−/− mice in the absence of added ligands, in the presence of 100 nM cytisine, and in the presence of 100 nM cytisine plus 50 nM α-conotoxin MII as indicated. Near adjacent sections were incubated with 0.5 nM [¹²⁵I] α-conotoxin MII. Labels: CP, caudate putamen; Cx, cerebral cortex; nAcc, nucleus accumbens; OT, olfactory tubercles; Pir, piriform cortex