Table 1. Primers used to amplify the individual exons of FRMD7.
| Exon | Forward primer | Reverse primer | Tm (°C) | Product length (bp) |
|---|---|---|---|---|
| 1 |
AGGAGACTGCCCAGATGCTA |
GGGCTGTTCACAAATGACAA |
60 |
348 |
| 2 |
GAAACAGGGCTTGCAGAGAG |
GCAATGCTAGACACAAAGAACC |
60 |
246 |
| 3 |
TGCTCAATGCCATGCTCTAC |
AAAGCCCTTTTCTCCCCTTA |
58 |
199 |
| 4 |
TGGGTGTGTGTGTGTGTGTG |
GCCCCCAATAAATGGAGAAT |
58 |
237 |
| 5 |
TGGATCTGGGAGAAGGAAAA |
GCTCCTGTGCTTGGTTCTCT |
60 |
238 |
| 6 |
GAGGACAAGGGTATGCTGGA |
TCAGGTTTAAGGGCTTGCTC |
58 |
281 |
| 7 |
GAGCTCTCAGGGTGGAAATG |
ACACCCAAGTTTGAGCCAAG |
58 |
293 |
| 8 |
TGCACTGTCTTACAAGCCAACT |
CGATTTGCAGAAACAACCAA |
56 |
231 |
| 9 |
TTGGGATTTGAAGGTCTTTGA |
TCCTCCTAAGCCTCCTGTGTT |
60 |
300 |
| 10-11 |
AGCCTATTGGTTTATGGCTAGAAC |
GCAGAATCAATTCATGGAAGC |
56 |
393 |
| 12a |
GGCCTTTTCCTTCTTTCACC |
CTGGGGAGGCATAATACCAA |
58 |
472 |
| 12b |
AGCTCCTTCCAAACAAGCTG |
TGACTGAGAGCAGGACAAGG |
60 |
524 |
| 12c | GCGGTAGGAGCAACATCAAT | CCAAGAAAATGGTTTCTACAACTTC | 58 | 560 |