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. 2011 Jan 31;11:45. doi: 10.1186/1471-2407-11-45

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Copyright ©2011 Gostner et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wnt signaling activity assay. Upon activation of Wnt signaling a luciferase emitted bioluminescence signal is measured. A reporter plasmid containing a constitutive active TCF/LEF motif determined the 100% activation value while a mutated motif lacking any consensus site for β-catenin binding represented the background luminescence level. Hs578T^EpCAMcells showed only a modest activation, whereas EpCAM overexpression in MDA-MB-231^EpCAMcould activate Wnt signaling about 20% in comparison to their empty vector controls.