Table 1.
Primer sequences used for real-time PCR
| Gene | GenBank Acc. Nr. | sequence (5'to 3') |
|---|---|---|
| TBP | NM_003194 | fwd TGCACAGGAGCCAAGAGTGAA |
| rev CACATCACAGCTCCCCACCA | ||
| EpCAM | NM_002354 | fwd CGCAGCTCAGGAAGAATGTG |
| rev TGAAGTACACTGGCATTGACG | ||
| TCF7L2 | NM_030756 | fwd TGCACTGTCCAGAGAAGAGC |
| rev GCTGCTTGTCCCTTTTCCTC | ||
| fwd TCAATGAATCAGAAACGAATCAA | ||
| rev CTCTTGGCCGCTTCTTCC | ||
| SFRP1 | NM_003012 | fwd GAGTTTGCACTGAGGATGAAAA |
| rev GCTTCTTCTTCTTGGGGACA | ||
| ITF-2 | NM_001083962 | fwd AGCCATTCTCTTCTGCCAAA |
| NM_003199 | rev CAGGTTCTCATCACCCTCGT | |
| CCND1 | NM_053056 | fwd GCTGCTCCTGGTGAACAAGC |
| NM_001758 | rev TTCAATGAAATCGTGCGGG | |
| MYC | NM_002467 | fwd CTACGCAGCGCCTCCCTCCACT |
| rev GGCGCTCCAAGACGTTGTGTGTTC |