. 2011 Jan 31;11:45. doi: 10.1186/1471-2407-11-45

Table 2.

Real-time PCR

	Expression of
	TBP		SRFP1		TCF7L2		ITF-2
n = 3	Ct ± SD	CV[%]	Ct ± SD	CV[%]	Ct ± SD	CV[%]	Ct ± SD	CV[%]
MDA-MB-231^control	22.11 ± 1.90	4.95	31.42 ± 2.90	9.22	19.19 ± 1.45	7.54	23.47 ± 0.43	1.82
MDA-MB-231^EpCAM	21.81 ± 0.10	0.47	34.37 ± 1.80	5.25	20.75 ± 0.27	1.33	36.26 ± 5.50	15.18
	comparison EpCAM expressing versus control
Regulation Factor			-11.06		-3.64		-1773.84
REST analysis
P(H1)			0.000		0.000		0.000
Result			DOWN		DOWN		DOWN

	Expression of
	TBP		SRFP1		TCF7L2		ITF-2
n = 4	Ct ± SD	CV[%]	Ct ± SD	CV[%]	Ct ± SD	CV[%]	Ct ± SD	CV[%]
Hs578T^control	23.23 ± 0.72	3.09	25.35 ± 2.07	8.17	21.28 ± 0.86	4.04	22.73 ± 1.81	7.96
Hs578T^EpCAM	22.54 ± 0.18	0.81	28.50 ± 3.39	11.91	20.76 ± 0.56	2.71	23.42 ± 0.54	2.30
	comparison EpCAM expressing versus control
Regulation Factor			-35.82		-0.73		-3.55
REST analysis
P(H1)			0.048		0.658		0.128
Result			DOWN		not different to control		not different to control

Quantification of differential mRNA expression of the Wnt signaling components SFRP1, TCF7L2 and ITF-2 in the stable EpCAM expressing cells in comparison to the empty vector control lines. (SD = standard deviation, CV = coefficient of variation, REST P(H1) = hypothesis test p value, significant if < 0.05).