Table 3.
Bath | Pipette | ||||||||
---|---|---|---|---|---|---|---|---|---|
Solution | NaCl-rich | Na-rich | K-rich | Li-rich | NMDG-rich | KCl-rich | Na-rich | K-rich | NMDG+Na |
B1 | B2 | B3 | B4 | B5 | P1 | P2 | P3 | P4 | |
NaCl | 150 | 10 | |||||||
KCl | 4 | 140 | |||||||
MgCl2 | 1 | 1 | |||||||
CaCl2 | 0.7 | 0.273 | |||||||
glucose | 5 | 5 | 5 | 5 | 5 | ||||
HEPES | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
EGTA | 1 | 1 | 1 | 1 | |||||
Na-ms* | 150 | 150 | 15 | ||||||
K-ms* | 150 | 150 | |||||||
Li-ms* | 150 | ||||||||
NMDG-ms* | 150 | 135 | |||||||
Ca-gluconate | 0.7 | 0.7 | 0.7 | 0.7 | 0.367 | 0.367 | 0.367 | ||
MgSO4 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
adjusted by | NaOH | NaOH | KOH | LiOH | NMDG | KOH | NaOH | KOH | NMDG |
pH | 7.4 | 7.4 | 7.4 | 7.4 | 7.4 | 7.2 | 7.3 | 7.3 | 7.3 |
*ms: Methanesulfonate