Skip to main content
. Author manuscript; available in PMC: 2012 Jan 7.
Published in final edited form as: J Proteome Res. 2010 Dec 13;10(1):320–329. doi: 10.1021/pr100953b

Figure 2.

Figure 2

(a) PPant ejection during MS2 generates characteristic ejection ions and charged loss parent peptides (z-1). The PPant ejection ion (m/z 318) can be further fragmented in MS3 to generate a characteristic signature, allowing unambiguous detection of PPant peptides. (b) Impact of CID energy applied during MS2 on PPant ejection. (c) Mechanism of enrichment of PPant peptides by FP-biotin 1. RG1 = fluorophosphonate, which reacts covalently with the conserved serine residue of PKS/NRPS TE domains, to allow active site enrichment.