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. Author manuscript; available in PMC: 2011 Feb 21.
Published in final edited form as: J Immunol. 2008 May 1;180(9):6176–6185. doi: 10.4049/jimmunol.180.9.6176

FIGURE 2.

FIGURE 2

HD5 and HD6 did not affect cell proliferation or HIV infection after viral entry. A, PHA-activated CD4+ T cells were incubated with HD5 or HD6 at various concentrations for 24 h. Cell viability was measured by Promega CellTiter 96 aqueous MTS assay. No difference was observed in cells in the absence or presence of HD5 or HD6 (p > 0.05). (B and C) To determine the effect of HD5 and HD6 on HIV infection after viral entry, HeLa-CD4-CCR5 cells or PHA-activated primary CD4+ T cells were infected with pseudotyped HIVVSV reporter virus for 2 h. Cells were washed and treated with HD5 or HD6 at various concentrations for 48 h before measuring luciferase activity. No difference was found between the control (without treatment) and defensin-treated samples (p > 0.05). Data are means ± SD of triplicate samples and represent three independent experiments. R.L.U., reflective light units.