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. Author manuscript; available in PMC: 2011 Feb 21.
Published in final edited form as: J Immunol. 2008 May 1;180(9):6176–6185. doi: 10.4049/jimmunol.180.9.6176

FIGURE 7.

FIGURE 7

A and B, siRNAs targeting HD5 or HD6 diminished GC-mediated enhanced HIV infectivity. Vaginal epithelial cells were transiently transfected with siRNAs targeting HD5 or HD6. Cells without transfection or with transfection of control siRNAs (control) were also included. After overnight incubation, cells were washed, exposed to GC, and cultured in fresh medium for 48 h. Conditioned medium were collected for HIV infection assay, whereas total RNAs were prepared for analyzing expression of HD5, HD6, and GAPDH by RT-PCR. When comparing the HIV enhancing effect of conditioned medium from untransfected cells or control siRNA-transfected cells, the difference between samples without and with GC exposure was significant (*, p < 0.05). However, when compared with the effect of conditioned medium from cells transfected with HD5 or HD6 siRNA, there was no difference between samples without or with GC exposure (p > 0.05). R.L.U., reflective light units.