Table I. HD5 and HD6 enhance infection of HIV primary isolatesa.
| Envelope | HD5 | HD6 |
|---|---|---|
| 92UG029(A), X4 | 1.68 ± 0.12 fold | 5.2 ± 1.04 |
| 92HT599(B), X4 | 1.7 ± 1.3 | 6.4 ± 0.9 |
| 92UG024(D), R5 | 7.2 ± 0.2 | 25 ± 4.8 |
| 92IN905(C), R5 | 8.5 ± 0.8 | 13.8 ± 0.8 |
| 92BR019(B), R5 | 39 ± 1.31 | 157 ± 9.1 |
| 92UG031(C), R5 | 69 ± 2.88 | 42.2 ± 3.9 |
| 92RW009(C/A), X4R5 | 145 ± 34 | 117 ±62 |
a
HIV-1 primary isolates (10 ng of p24 per sample) with various subtypes and coreceptor usages were incubated with HD5 or HD6 at 20 μg/ml at 37°C for 1 h before addition to TZM- bl indicator cells for 2 h. Cells were washed and luciferase activities were measured 48 h after infection. The data are presented as fold increase compared to untreated samples in triplicate. Results represent two independent experiments (mean ± SD). The subtypes are shown in parentheses.