Hippocampal cultures were fixed 6 hours (A-D), 1 day (E-H) or 4 days (I-L) after plating and stained for Tmod2 (A, E and I), F-actin (B, F and J), and β3-tubulin (C, G and K). High magnification insets are displayed as merges (D, H and L). Shown are representative cells from the respective time points. Magnification of insets 1.5× in A-C, 2× in E-G and 2× in I-K. Scale bars = 10μm except for D, H, and L where scale bars = 5μm.