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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Nov 18;1812(4):565–571. doi: 10.1016/j.bbadis.2010.11.003

Figure 5. TNF-α treatment decreases HcrtR2 expression in B35 cells transfected with MSCV-HcrtR2 vector.

Figure 5

B35 cells overexpressing the HcrtR2 (mediated by MSCV-HcrtR2) were cultured in the presence and absence of TNF-α cytokine (2 ng/ml) for 24 hours at 37°C. Panel A: Cells were lysed, and equivalent amount of whole cell detergent lysates were Western blotted with the indicated antibodies. Panel B: Densitometry of HcrtR2 expression as in Fig. 2. Panel C: mRNA level of HcrtR2 was analyzed by quantitative real time RT-PCR as in Fig. 2. Data are presented as mean ± S.D. (n = 4). * represents p < 0.01 for TNF-α-treated cells compared to vehicle-treated control group.