Hoe 140 blocks the BK-stimulated increase in CGRP mRNA accumulation (panel A) and iCGRP release (panel B). Neuronal cultures were treated for 24 hours with either Hoe 140 (10 μM) alone, BK (1 μM) alone, or a combination of BK and Hoe 140 (n=3 in triplicate). The BK2 receptor antagonist was added 30 minutes before the BK and was maintained in the medium throughout the incubation. Control cells were treated with vehicle. CGRP mRNA content and iCGRP levels in the medium were determined as described previously and the values are expressed as fold-induction over control. * p < 0.05, BK vs. control, BK + Hoe 140, Hoe 140.