Figure 3.
Biliverdin reductase-A (BVR-A) phosphorylation on Tyrosine (Tyr) residues in hippocampus and cerebellum of Alzheimer diseases (AD) and mild cognitive impairment (MCI) subjects. Brain samples of hippocampus (panels A and B) and cerebellum (panels C and D) of subjects with AD or MCI were immunoprecipitated by using an anti-BVR-A antibody and assayed for phospho-Tyr (pTyr) by Western Blot as described under Materials and Methods (sections 2.4, 2.5 and 2.7). Densitometric values shown in the histograms are given as percentage of Control, set as 100%, and are the product of the band value of the levels of pTyr-BVR-A normalized per total BVR-A as loading control. In panels A-D representative gels are shown. Data are expressed as mean ± SD of six individual samples per group. *P < 0.05 and **P < 0.01 versus Control (Student's t-test).