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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Neuroendocrinol. 2011 Mar;23(3):216–223. doi: 10.1111/j.1365-2826.2010.02101.x

Fig 4.

Fig 4

Restraint stress induces recruitment of phospho-CREB and TORC2 by the CRH promoter in the hypothalamic PVN region. ChIP assays using phospho-CREB and TORC 2 antibodies and cross-linked DNA from microdissected hypothalamic PVN region of control rats and rats subjected to restraint stress for 30min or 3h after 1h restraint (240 min) (A). Amounts of CRH promoter DNA in the immunoprecipitant was measured by qRT-PCR. Bars represent the mean and SE of the results obtained in 4 experiments using pooled tissue from 2 or 3 rats per experimental point. Gel shows the results obtained in pools of hypothalamic tissue from 3 rats per group in a representative experiment (B). **, p< 0.01 higher than basal; **, p<0.02 higher than basal; #, p<0.02 lower than 30 min.