Table 1. Peptides showing cysteine S-nitrosylation as identified via SNO-RAC proteomic analysis.

Protein Name	Protein ID	Peptide Sequence	SNO Cys	Perfusion		IPC		IR		IPC-IR
				Ratio	Ion Score	Ratio	Ion Score	Ratio	Ion Score	Ratio	Ion Score
Citrate synthase (M)	Q9CZU6	GYSIPECQK	101	1	37	3.29	30	0.43*	30
Cytochrome b-c1 complex subunit 1 (M)	Q9CZ13	VYEEDAVPGLTPCR	268				79
		LCTSATESEVTR	380	1	81	7.31*	77			3.30*	92
Fructose-bisphosphate aldolase A	P05064	ALANSLACQGK	339	1	83	9.90*	90	1.70*,†	80	10.00*,‡	81
Glyceraldehyde-3-phosphate dehydrogenase	P16858	IVSNASCTTNCLAPLAK	150,154	1	124	2.97*	122	0.07*,†	126	1.34†,‡	129
		VPTPNVSVVDLTCR	245	1	105	2.10*	98			0.90†	101
Isocitrate dehydrogenase [NADP] (M)	P54071	SSGGFVWACK	308				62
		VCVQTVESGAMTK	402	1	116	1.91	112			1.01†	109
L-lactate dehydrogenase A chain	P06151	IVSSKDYCVTANSK	84				51
		VIGSGCNLDSAR	163	1	96	3.81*	86
Malate dehydrogenase	P14152	VIVVGNPANTNCLTASK	137	1	113	0.61*	126			0.70	115
Malate dehydrogenase (M)	P08249	EGVVECSFVQSK	275	1	60	1.16	76			1.22	67
Protein NipSnap homolog 2	O55126	ICQEVLPK	89	1	55	22.40*	53	0.30*,†	54	9.91*,†,‡	58
Succinyl-CoA ligase α (M)	Q9WUM5	IICQGFTGK	60	1	40	0.71	48	0.23*,†	43	0.63	45
Triosephosphate isomerase	P17751	IAVAAQNCYK	67	1	59	3.40*	62	0.44*,†	53	1.42*,‡	43
		IIYGGSVTGATCK	218				103				107
Aconitate hydratase (M)	Q99KI0	VGLIGSCTNSSYEDMGR	385				124				102
Aspartate aminotransferase	P05201	INMCGLTTK	391				57
Aspartate aminotransferase (M)	P05202	VGAFTVVCK	295				44				48
β-enolase	P21550	VNQIGSVTESIQACK	357				101
Cysteine and glycine-rich protein 3	P50462	TVYHAEEIQCNGR	25				73				41
Cysteine-rich protein 2	Q9DCT8	ASSVTTFTGEPNMCPR	126				76
Cytochrome c oxidase subunit 5b (M)	P19536	cPNCGTHYK	115				30
Electron transfer flavoprotein subunit α (M)	Q99LC5	TIYAGNALCTVK	155				80
Electron transfer flavoprotein subunit β (M)	Q9DCW4	EIIAVSCGPSQCQETIR	66,71				103
Enoyl-CoA hydratase (M)	Q8BH95	LVEEAIQCAEK	225				80
Isocitrate dehydrogenase [NAD] subunit α (M)	Q9D6R2	IEAACFATIK	331				30
Long-chain-fatty-acid-CoA ligase 1	P41216	GIQVSNNGPCLGSR	109				72
Metallothionein-1	P02802	SCCSCCPVGCSK	33,34,36 37,41				45
		GAADKCTCCA	57,59,60				49
NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 10 (M)	Q99LC3	VITVDGNICSGK	67				69
Peroxiredoxin-6	O08709	DFTPVCTTELGR	47				44
Propionyl-CoA carboxylase α chain	Q91ZA3	MADEAVCVGPAPTSK	107				95
Succinate dehydrogenase [ubiquinone] flavoprotein (M)	Q8K2B3	VGSVLQEGCEK	536				51
Succinyl-CoA ligase subunit β (M)	Q9Z2I9	ILACDDLDEAAK	430				68
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2	O55143	SMSVYCTPNKPSR	498								44
Tubulin β-4 chain	P04350	NMMAACDPR	303				48				49
Very long-chain specific acyl-CoA dehydrogenase (M)	P50544	SSAIPSPCGK	238								35
Voltage-dependent anion channel protein 2 (M)	Q60930	SCSGVEFSTSGSSNTDTGK	48				83				86

Each protein/peptide was identified from at least three of five SNO-RAC/LC-MS/MS proteomic analyses (peptides were filtered at a false discovery rate of 5%; peptides with ion scores below 30 were not accepted). (M): mitochondrial isoform; (C): SNO cysteine residue; (c): NEM-blocked cysteine residue; Peptides not detected under the specified condition contain a blank space in the ion score column.

^*p<0.05 vs. Perfusion;

^†p<0.05 vs. IPC;

^‡p<0.05 vs. IR.