Figure 3.

Probing specific motor protein activity using inducible intracellular motility assays. (A–F) Peroxisome distribution before (A–C) and after (D–F) rapalog addition in the presence of KIF1A (A and D), BICDN-FRB (B and E), or Myosin Vb (C and F). (G–I) Overlay of sequential binarized images from the recordings in A–F, color-coded by time as indicated in G. Blue marks the initial distribution, and red marks regions targeted upon addition of rapalog. (J–L) Time traces of the R_90% (radius of circle enclosing 90% of total cellular PEX intensity; see Fig. 2) for the indicated constructs. Rapalog is added at 0:00 min. Scale bars: 10 μm.