Figure 4.

Chemical cross-linking of the truncated hGBP-1 proteins. This was done in the presence of 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride and n-hydroxy succinimide as described in the Materials and Methods in the Supporting Material and the products were run on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The experiments were done both in the absence and presence of the cross-linker. The experiments were carried out in duplicate and the results were highly reproducible.