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GTPase assays of the unimmobilized and immobilized proteins. The assay was carried out by mixing the proteins with a trace amount of [α-32P] GTP plus 50 μM of unlabeled GTP at 37°C for 1 h. The reaction was terminated with 250 mM EDTA (final concentration). The reaction mixture was separated as described earlier. These experiments were carried out in triplicate and the results were reproducible.
