Table 1.
Molecular masses and dimerization status of the wild-type, mutant, and truncated proteins in the presence and absence of GTP-analog GppNHp based on the analytical size-exclusion chromatography
| Protein | Molecular mass with GppNHp (kDa) | Molecular mass without GppNHp (kDa) | Dimer/monomer with GppNHp | Ability to produce GMP |
|---|---|---|---|---|
| whGBP-1 | 135 ± 4.5 | 78 ± 2 | Dimer | Yes |
| D108A | 135 ± 4 | 78 ± 2.5 | Dimer, Monomer | Yes |
| D103L.D108L | 79 ± 3 | 78 ± 2 | Monomer | No |
| hGBP-1317 | 62.5 ± 3 | 34.6 ± 2.5 | Dimer | Yes |
| hGBP-1311 | 62.5 ± 4 | 34.6 ± 3 | Dimer | Yes |
| hGBP-1307 | 62.5 ± 3 | 34.6 ± 2.5 | Dimer | Yes |
| hGBP-1289 | 34.6 ± 3 | 34.6 ± 2.5 | Monomer | No |
| hGBP-1278 | 34.6 ± 3 | 34.6 ± 3 | Monomer | No |
| hGBP-1279-592 | ND | 143 ± 3, 46 ± 2 | Dimer, Monomer | — |
| hGBP-1290-592 | ND | 143 ± 3, 46 ± 2.5 | Dimer, Monomer | — |
| hGBP-1308-592 | ND | 46 ± 2 | Monomer | — |
| hGBP-1312-592 | ND | 46 ± 2 | Monomer | — |
| hGBP-1318-592 | ND | 46 ± 2 | Monomer | — |
Experiments were carried out in triplicate and the results were found to be consistent. ND, not determined.