pH titrations performed in the E1 (A) and P-E2 (B) conformations, in the absence (solid circles) and presence (open circles) of 10 μM free Pb2+. Experimental data were fitted by Eq. 2. (A) pK = 6.8 ± 0.1, n = 0.9 ± 0.2 (no Pb2+); pK = 6.56 ± 0.08, n = 1.2 ± 0.3 (with Pb2+). (B) pK = 5.57 ± 0.02, n = 1.00 ± 0.04 (no Pb2+); pK = 5.87 ± 0.09, n = 1.1 ± 0.2 (with Pb2+).