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Dependence of the steady-state fluorescence levels of states HxE1 (A) and P-E2 (B) on Pb2+ concentration. (Solid lines) Binding isotherms describing the equilibria Pb2+ + HxE1 + yH+ ⇆ Hx+yE1Pb (A) and Pb2+ + P-E2 + zH+ ⇆ P-E2Pb (Hz) (B). Best fitting with Eq. 1 gives the following values: (ΔF/F0)0 = 0, (ΔF/F0)max = −0.04 ± 0.01, K0.5 = 3.1 ± 0.9 μM, and n = 2 ± 1 (A); (ΔF/F0)0 = 0.187 ± 0.006, (ΔF/F0)max = −0.13 ± 0.02, K0.5 = 2.5 ± 0.4 μM, and n = 2.1 ± 0.7 (B).
