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Effect of the A322D mutation on the GABAA receptor α1 subunit. (A) ρTM at kBT set at 0.01 kcal/mole. (Dashed line) Mutant. (B) ρTM at room temperature. (Dashed line) Mutant. (Inset) Occupancy of the mutant at the value of μ = 0.34 kcal/mol (arrow). The mutation occurs in the third TM segment.
