Figure 2.

Aβ causes cholesterol sequestration in primary neurons. (a) Rat primary neurons (forebrain and sympathetic) cultured in serum-free medium, were treated for 24 h with 20 μM oligomeric Aβ₄₂ (prepared according to [66]) or with 1.5 μM U18666A, a drug extensively used to induce cholesterol sequestration. Cholesterol was examined by confocal microscopy, using filipin. (b) Neurons were treated as in (a) but fluorescent oligomeric Aβ₄₂ was used. Intracellular localization of cholesterol and Aβ was examined by double indirect immunofluorescence confocal microscopy using LAMP1 as a marker of late endosome/MVBs and EEA1 as a marker of early endosomes.