|
Tris-HCL (50 mM) pH 7.5 |
The liver biopsy was homogenized in 20 volumes of cold buffer, and then the supernatant was harvested after centrifugation at 5000 g for 30 min at 4°C. |
SOD, CAT and GSH |
[24, 25] |
|
Chilled potassium chloride (1.17%) |
Liver biopsy was homogenized in chilled buffer. The homogenates were centrifuged at 800 g for 5 min at 4°C to separate the nuclear debris. The obtained supernatant was recentrifuged at 10,500 g for 20 min at 4°C to get the postmitochondrial supernatant. |
SOD, CAT and MDA |
[68] |
Liver biopsy samples were washed twice in cold 0.9% salt solution |
Ice-cold PBS buffer (20 mM), pH 7.3 with 10 ml of 5 mM butylated hydroxyl toluene |
The tissue was homogenized in 290 ml ice-cold buffer. Following this, the suspension was centrifuged and supernatant was fractioned for analysis. |
LPO and AOP |
[69] |
|
Tris-HCl (50 mM), pH 7.5, 5 mM EDTA, 1 nM dithiothreitol |
The tissue was homogenized in 5 ml/g cold buffer. The homogenate was centrifuged at 10,000 g for 15 minutes at 4°C. The supernatant was removed for assay. |
GSH-Px |
[70] |
|
Potassium phosphate (0.05 M) and 0.1 mM EDTA, pH 7.8 |
The tissue was homogenized in 200 μL buffer and centrifuged at 15,000 g for 30 minutes at 4°C. The supernatant was used for analysis. |
SOD |
[70] |