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. 2011 Feb 10;2011:632705. doi: 10.4061/2011/632705

Figure 1.

Figure 1

Agarose gel electrophoresis (1%) of the cloned fragment of the gene encoding PPE protein in pIRES IFN PPE mammalian vector. Lane M1: DNA molecular weight marker Lamda DNA/EcoRI/Hind III. Lane 1: pIRES IFN PPE linearised with EcoRI. Lane 2: released insert of 1080 bp after NheI and EcoRI digestion of recombinant pIRES IFN PPE recombinant plasmid DNA. Lane 3: PCR amplified fragment encoding PPE protein of Mycobacterium avium subsp. paratuberculosis. Lane 4: pIR IFN linearised with Xba I. Lane 5: release IFN fragment (467 bp) by digestion with Xba I and Not I from pIR IFN vector DNA. Lane 6: release IFN fragment (467 bp) by digestion with Xba I and Not I from pIR IFN PPE vector DNA. Lane 7: PCR amplified fragment of IFN (467 bp). Lane M2: DNA molecular weight marker pUC18/Sau3AI-pUC18/TaqI digest.