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Agarose gel electrophoresis (1.5%) showing RT-PCR based expression of IFNγ ORF in pIR PPE IFN transfected HeLa cells. Lane M: prestained protein molecular weight marker (100 bp ladder). Lane 1: negative control mock plasmid transfected HeLa cells. Lane 2: IFNγ encoding gene from pIR PPE IFN transfected HeLa cells with super natantγ. Lane 3: positive control (IFNγ) encoding gene from ConA induced mice splenocytes.
