Figure 2.

Glutamine depletion induces severe damage of the colonic epithelial structure with reduced epithelial cell growth. A: Representative pictures of H-E staining (left panels) and immunohistochemical staining for BrdU (middle panels) and Ki-67 (right panels). Each colonic epithelium was taken from infant mice fed with glutamine-rich milk (GRM), complete amino acid milk (CAM), or glutamine-deleted milk (GDM). Magnification, × 200. Positive stained epithelial cells with BrdU (B) and Ki-67 (C) were counted and compared with each group in a histogram. ^bP ≤ 0.001; ^cP < 0.05.