Figure 5.

Potent antiproliferative effect of glutamine depletion results in increased cleavage of caspase-3. Cell cycle distribution under culture condition with different concentrations of glutamine was analyzed by flow cytometry (A) and cell populations at the sub-G0 phase were compared with each other (B); C: Immunoblotting for caspase-3 and cleaved caspase-3 revealed the induction of apoptosis in IEC6 cells after 24 h of culture without glutamine. Each experiment was independently repeated three times and the representative data among the similar results are shown.