Table 1.
Representative subsets of differentially expressed mast-cell genes after bacterial stimulation classified into functional categories
| Go class | Description |
P value |
Gene examples | |||||
| LGG | Lc705 | PJS | Bb12 | Combination | Cpn | |||
| Upregulation | ||||||||
| GO:0002376 | Immune system process | 3.48E-16 | 2.95E-13 | - | 5.25E-7 | 5.66E-12 | 1.64E-4 | TNF, IL1B, IL8, IL10, CCL2, TLR1, TLR6, NOD2 |
| GO:0043067 | Regulation of programmed cell death | 2.77E-8 | 9.63E-8 | - | 7.18E-4 | 3.49E-6 | - | CASP3, CASP8, CDKN1 |
| GO:0045321 | Leukocyte activation | 8.97E-9 | 2.17E-4 | - | - | 2.23E-4 | - | CD8A, LCP2 |
| Downregulation | ||||||||
| GO:0007049 | Cell cycle | 4.76E-5 | 9.61E-7 | - | - | 5.81E-4 | - | CCNA2, MAPK12 |
| GO:0033033 | Regulation of mast cell activation | - | 7.51E-4 | - | - | - | - | FCER1A, FCER1G |
| GO:0008610 | Lipid biosynthetic process | - | - | - | 2.02E-5 | - | - | LPGAT1 |
The three selected highly upregulated or downregulated functional categories from the enrichment analysis performed with GOrilla Gene Enrichment Analysis and Visualization Tool. P value is the enrichment P value reported by GOrilla. The resultant categories reflect the gene expression differences of the bacteria-stimulated sample compared to unstimulated sample in 24 h time point. LGG: Lactobacillus rhamnosus GG; Lc705: Lactobacillus rhamnosus Lc705; PJS: Propionibacterium freudenreichii ssp. shermanii JS; Bb12: Bifidobacterium animalis ssp. lactis Bb12; Cpn: Chlamydia pneumoniae isolate Kajaani 6; TNF: Tumor necrosis factor; IL-1B: Interleukin-1β; IL-8: Interleukin-8; IL-10: Interleukin-10; CCL2: Chemokine (C-C motif) 2; TLR1: Toll-Like receptor 1; TLR6: Toll-Like receptor 6; NOD2: Nucleotide-binding oligomerization domain-containing protein 2; CASP3: Caspase 3; CASP8: Caspase 8; CDKN1: Cyclin-dependent kinase inhibitor 1; CD8A: Cluster of differentiation 8 A (T cell surface glycoprotein); LCP2: Lymphocyte cytosolic protein 2; CCNA2: Cyclin A2; MAPK12: Mitogen-activated protein kinase 12; FCER1A: Fc fragment of IgE high affinity I receptor for α polypeptide; FCER1G: Fc fragment of IgE high affinity I receptor for γ polypeptide; LPGAT1: Lysophosphatidylglycerol acyltransferase 1.