Skip to main content
. Author manuscript; available in PMC: 2012 Feb 2.
Published in final edited form as: Cell Metab. 2011 Feb 2;13(2):215–221. doi: 10.1016/j.cmet.2011.01.007

Figure 3. Human IPMK-1–60 peptide interferes with mTOR-raptor binding and amino acid signaling.

Figure 3

(A) HEK293T cells transfected with GFP or GFP-hIPMK-1–60 were deprived of serum and leucine for 1 hour, and stimulated with leucine for 10 min. mTORC1 and mTORC2 were isolated in the presence of DSP, and the amount of mTOR was analyzed. (B) S6K phosphorylation and protein expression were analyzed by immunoblotting. (C) The amount of myc-raptor bound in mTOR pulldown and in vitro mTORC1 activity were analyzed. mTOR immunopurified from leucine-treated HEK293T cells and myc-raptor (100 nM) were co-incubated with GST or GST-hIPMK-1–60 (200 nM). Bars represent mean ± SD (n = 3 in A and C, n = 4 in B). *p < 0.05; **p < 0.01.