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. Author manuscript; available in PMC: 2012 Jan 7.
Published in final edited form as: Mol Cell. 2011 Jan 7;41(1):93–106. doi: 10.1016/j.molcel.2010.12.004

Figure 5. Most GAD fusions are San1 substrates.

Figure 5

(A, B, C) Cycloheximide-chase assays of cells expressing the indicated GAD fusion were performed to assess stability in the presence or absence of SAN1. GAD fusion expression was induced by addition of galactose for 3 hours prior to cycloheximide addition. Time after cycloheximide addition is indicated above each lane. Anti-GAD antibodies were used to detect each GAD fusion. (D) 2xHA-tagged GAD substrates were immunoprecipitated from E. coli cells expressing the San1 ubiquitination cascade. Western blots were probed with anti-ubiquitin antibodies to assess substrate ubiquitination or anti-HA antibodies to assess substrate IP. Dashes above the lanes indicate which components were not expressed. Asterisk marks the antibody band.