Figure 7. Each substrate has a distinct degradation profile with the San1 deletions.
(A) Cartoon illustrating quartile scoring of steady-state effects. (B) Steady-state levels of the indicated substrate were examined by flow cytometry. The percent of normal San1 function was calculated by comparing each deletion steady-state level median with the median in SAN1 and san1Δ cells. Cycloheximide-chase assays of cells with each SAN1 deletion were performed to assess stability of San1. Western blots were probed with anti-HSV antibodies. Time after cycloheximide addition is indicated above each lane. (C) Model for how San1 uses the plasticity of disorder to interact with its substrates.