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. Author manuscript; available in PMC: 2011 Nov 24.
Published in final edited form as: Immunity. 2010 Nov 24;33(5):736–751. doi: 10.1016/j.immuni.2010.10.017

Figure 4. Human IL-22-producing cells depend on IL-7 for their development.

Figure 4

(A,B) Quantitative RT-PCR analysis of IL22 and RORC expression by the indicated, populations from human tonsils (A) or peripheral blood (B). Stage 1: CD34+CD117− CD94−, Stage 2: CD34+CD117+CD94−, Stage 3: CD34−CD117+CD94−, Stage 4: CD34−CD117+CD94+.

(C,D) Mononuclear cells were stained with antibodies specific for the indicated markers and for CD3 and CD19. Dot plots (C) were electronically gated on CD3−CD19− cells. Contour plots (D) exclude all CD3+ and CD19+ cells and are electronically gated on CD34+ (stage 1 and stage 2) or CD34− cells (stage 3 and stage 4). Numbers indicate percentage of cells within the adjacent gates. Data are representative of two independent experiments.