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Relocalization of La by HA-gg88. The 293T and NIH 3T3 cells were transfected with HA-gg88 and the JSRV Env-expressing vector ΔGP-HA (transfection control). The transfected cells were fixed at 36 to 48 h posttransfection with paraformaldehyde and then incubated with anti-HA and anti-La antibodies. The antigens were visualized by the secondary antibodies conjugated with Alexa 488 and Alexa 546. The panels for La, HA, and a merge (La-HA-DAPI) are shown.
