FIG 6 .
Interference of La expression antagonizes HA-gg88 in M-MuLV particle release. M-MuLV Gag-Pol expression vector is transfected into 293T cells with or without HA-gg88. At 24 hours posttransfection, siRNAs against human La (no. 5 and no. 10) were transfected. Two days after siRNA transfection, media were replaced, and then the cells and media were harvested after 8 h of further incubation. The same portion of cells and viruses were subjected to the Western blots with anti-p30CA, anti-La, and anti-β-Tubulin (loading control). NSP, non-specific signals.