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Overexpression of HA-gg88 and La increases the efficiency of HIV-1 particle release. The HIV-1 Gag-Pol expression vector was transfected into 293T cells with p8065-2 and HA-gg88 (A) or mouse La and human La expression vectors (B). The cells and media were harvested at 48 h posttransfection, and the same portion of cells and viruses were analyzed by Western blotting with anti-HIV p24CΑ and anti-β-Tubulin (loading control).
